Mutarotation was further investigated recording the time course of specific rotation [α] at 589 nm after the dissolution of D-psicose in water. Structure, properties, spectra, suppliers and links for: 6-O-Phosphonato-D-psicose. D-Allulose (D-psicose) GRAS notice Tables . Intended Use and Maximum Use Levels of D-allulose, % (w/w) 5 Table 2-1. Recently, a characterization study of a putative d-TE from Agrobacterium tumefaciens that can specifically catalyze the interconversion of d-fructose and d-psicose was reported. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. KW - Crystal structure Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. The cell volume of racemic β-D,L-psicose [763.21 (6) Å3] is almost the same as that of chiral β-D-psicose [753.06 Å3]. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. Draw The Structure Of D-psicose. The RCSB PDB also provides a variety of tools and resources. EC 5.1.3.31, D-tagatose 3-epimerase). The enzyme from the bacterium Clostridium scindens requires Mn2+ , whereas the enzymes from the bacteria Clostridium cellulolyticum [2,5], Clostridium sp. The substrate specificity decreases in the following order: D-fructose, D-tagatose, D-ribulose, D-xylulose, and D-sorbose. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. The enzyme showed maximum activity at pH 7.5 and 60°C. Question: D-Psicose, The C3 Epimer Of Fructose, Has No Calories And Has Also Been Found To Have Hypoglycemic Effects, Which Might Be Useful In Treating Diabetes. Links RCSB PDBe PDBj PDBsum CATH PLIP Citation Kim, K. et al., Crystal Structure of d-Psicose 3-epimerase from Agrobacterium tumefaciens and its Complex with True Substrate d-Fructose: A Pivotal Role of Metal in Catalysis, an Active Site for the Non-phosphorylated Substrate, and … The title compound, C6H12O6, a C-3 position epimer of fructose, was crystallized from an aqueous solution of equimolar mixture of d- and l-psicose (1,3,4,5,6-pentahydroxyhexan-2-one, ribo-2-hexulose, allulose), and it was confirmed that d-psicose (or l-psicose) formed β-pyranose with a 2 C 5 (or 5 C 2) conformation. Solubility of D-psicose at 25 °C was 291 g per 100 g water. It has been reported that d-psicose has a short half-life and is not metabolized in the body. d-Psicose, a C3 epimer of d-fructose, is known to lower body weight and adipose tissue weight and affect lipid metabolism. d-Psicose, a rare sugar produced by the enzymatic reaction of d-tagatose 3-epimerase (DTEase), has been used extensively for the bioproduction of various rare carbohydrates.Recently characterized d-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was found to belong to the DTEase family and to catalyze the interconversion of d-fructose and d-psicose by epimerizing the … d-Psicose 3-epimerase (DPEase) is demonstrated to be useful in the bioproduction of d-psicose, a rare hexose sugar, from d-fructose, found plenty in nature. The reduction in the dielectric relaxation strength due to starch retrogradation was more pronounced in the presence of sucrose or trehalose than D ‐psicose. Involved in the biosynthesis of D-psicose. The noncharacterized protein CLOSCI_02528 from Clostridium scindens ATCC 35704 was characterized as D-psicose 3-epimerase. English: Structure of D-psicose. X‐ray diffraction peaks arising from crystalline structures in starch disappeared upon heating up to 68°C in the presence of D ‐psicose and 75°C in the presence of sucrose or trehalose. D-psicose 3-epimerase (DPEase) is demonstrated to be useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature. Catalyzes the reversible epimerization of D-fructose at the C3 position to yield D-psicose. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. The structures of D-glucose and D-psicose are shown below. The enzyme is highly specific for D-psicose and shows very low activity with D-tagatose (cf. The columns are linked by other O - H⋯O hydrogen bonds between D- and L-psicose molecules, forming a three-dimensional network. The RCSB PDB also provides a variety of tools and resources. D‐Psicose (D ‐ribo‐2‐hexulose), a C‐3 epimer of D ‐fructose, is present in small quantities in commercial carbohydrate complexes or agricultural products.Wistar male rats were fed experimental diets which consisted of 5% D ‐psicose, cellulose, D ‐fructose or D ‐glucose for 28 days. Data: 17 de marzo de 2007: Orixe: Obra propia: Autor: NEUROtiker: Licenza (Reuso deste ficheiro) Own work, all rights released (Public domain) Public domain Public domain false false: Eu, como posuidor dos dereitos de autor … Catalyzes the reversible epimerization of D-fructose at the C3 position to yield D-psicose. Additional crystal structures of the enzyme in complex with substrates/products (D-psicose, D-fructose, D-tagatose and D-sorbose) were also determined. It was strictly metal-dependent and required Mn2+ as optimum cofactor for activity. 3VNK: Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. The formation of the psicose-borate complex was the result of the higher binding affinity of borate for psicose than for fructose. The conversion yield of d-psicose from d-fructose by a d-psicose 3-epimerase from Agrobacterium tumefaciens increased with increasing molar ratios of borate to fructose, up to a ratio of 0.6. Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize d-fructose to yield d-psicose with a much higher conversion rate when compared with the conventionally used DTEase. The enzyme is highly specific for D-psicose … explain your … This message will disappear when all data is loaded. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. What test could be used to distinguish between solutions of these two carbohydrates? It is thought that [α] for β-D-psicopyranose in water may be ca. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Composition of Product 1 11 Table 2-2. CH OH C=O CHO HO–C-H … D-psicose, a rare sugar produced by the enzymatic reaction of D-tagatose 3-epimerase (DTEase), has been used extensively for the bioproduction of various rare carbohydrates. 551-68-8 - BJHIKXHVCXFQLS-PUFIMZNGSA-N - D-Psicose - Similar structures search, synonyms, formulas, resource links, and other chemical information. An intramolecular O - H⋯O hydrogen bond is also observed. −85 deg dm −1 g −1 cm 3. The precise mechanism remains unknown. view of D-psicose (2077 - BJHIKXHVCXFQLS-PUFIMZNGSA-N) Please wait a moment until all data is loaded. Chemical structure of D-allulose is shown in Figure 1. BNL1100 , and Clostridium bolteae require Co2+ as optimum cofactor. Click And Hold Each Term To Drag It To Its Appropriate Location. Crystal structure of b-D,L-psicose Tomohiko Ishii,a* Genta Sakane,b Akihide Yoshihara,c ... confirmed that d-psicose (or l-psicose) formed -pyranose with a 2C 5 (or 5C 2) conformation. To determine how d-psicose modifies lipid metabolism, rats were fed diets with or without 3% d-psicose for 4 weeks. Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher con … Figure 1. Crystal Structure of d-Psicose 3-epimerase from Agrobacterium tumefaciens and its Complex with True Substrate d-Fructose: A Pivotal Role of Metal in Catalysis, an Active Site for the Non-phosphorylated Substrate, and its Conformational Changes Kim, K., Kim, H.J., Oh, D.K., Cha, S.S., Rhee, S. (2006) J Mol Biol 361: 920-931 The title compound, C6H12O6, a C-3 position epimer of fructose, was crystallized from an aqueous solution of equimolar mixture of d- and l-psicose (1,3,4,5,6-penta-hydroxy-hexan-2-one, ribo-2-hexulose, allulose), and it was confirmed that d-psicose (or l-psicose) formed β-pyran-ose with a (2) C 5 (or (5) C 2) conformation. These results indicate that the ligand binding may not induce significant conformational change for overall structure. Although d-psicose presents in small amounts in natural products, a recent new technique using biocatalyst sources enables large-scale d-psicose production. Table 1. More importantly, several clinical and animal studies demonstrated that d -psicose has hypoglycemic, hypolipidemic, and antioxidant activities, which make it an ideal candidate for preventing diabetes and related health concerns. structures in complex with D-psicose, D-fructose, D-tagatose and D-sorbose are 0.183 Å, 0.171 Å, 0.186 Å and 0.197 Å, respectively, for all Cα atoms. The enzyme is highly specific for D-psicose and shows very low activity with D-tagatose. Involved in the biosynthesis of D-psicose. The half-life of the enzyme at 50°C was 108 min, suggesting the enzyme was relatively thermostable. ... M. Rare sugar D-psicose improves insulin sensitivity and glucose tolerance in From the complex structures of C. cellulolyticum DPEase with D-psicose and D-fructose, the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues. 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